Fig. 5.

Effect of coculture of myotubes with NG108-15 cells on nPKC θ expression. Primary myoblast cultures were established and cultured for 7 d as described in the legend to Figure 2. Myotubes were then cocultured for 2.5 d in the presence of the indicated number of NG108-15 cells. The cultures were homogenized and centrifuged at 6000 × g for 2 min. Proteins contained in equal representative volumes from each supernatant were separated by SDS-PAGE, transferred to nitrocellulose membranes, and analyzed according to the legend to Figure 1. Data are expressed as percent increase in nPKC θ expression compared with parallel myotubes cultured in NG108-15 cell differentiation medium without NG108-15 cells. For each point, n = 1.