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. 1996 Aug 15;16(16):4949–4957. doi: 10.1523/JNEUROSCI.16-16-04949.1996

Fig. 4.

Fig. 4.

a, Transcriptional start site mapping of the myomodulin gene. Three products of reverse transcription of total CNS RNA from the radiolabeled primer MMRTA1 were separated by PAGE (lane i) using the ddCTP reaction of a manual sequencing reaction as a size marker (lane C). The sequence of genomic clone pGMM28A to which the marker corresponds is shown on the right (cytosines are underlined). The nearest adenosines to which the reverse transcription products could correspond are marked with arrows (the faint largest product is marked by a gray arrow). The first base of the least truncated cDNA clone isolated is marked with anasterisk. b, Northern blot of total CNS RNA hybridized with a radiolabeled class III myomodulin cDNA. Six bands of 3.8, 3.2, 2.8, 2.6, 2.1, and 1.5 kb (indicated with arrows) were detected. Three of these correspond to sizes predicted for the three classes of myomodulin cDNA isolated (2.1, 2.6, and 2.8 kb).