Figure 3.

Suppression of type III IFN signaling by HRTV infection and HNSs expression. A, HRTV infection interferes with IFN-λ–stimulated ISRE activation. Huh7 cells transfected with the reporter plasmids were mock-infected or infected with HRTV or SFTSV for 24 h. Cells were then stimulated with IFN-λ (100 ng/ml) for 18 h or left unstimulated, before the measurement of luciferase activities. B, HNSs functions as an antagonist of type III IFN signaling. Huh7 cells were transfected with the reporter plasmids, along with the vector plasmid or the plasmids encoding SNSs or HNSs. At 24 h posttransfection, cells were left untreated or treated with IFN-λ (100 ng/ml) for 18 h, followed by the detection of luciferase activities. Graphs show mean ± S.D. (error bars) (n = 3). *, p < 0.05; **, p < 0.01.