Figure 6.

HNSs specifically inhibits nuclear translocation of STAT2 (but not STAT1), in contrast to SNSs inhibition of both STAT2 and STAT1 nuclear accumulation. HEK293 cells were transfected with the HNSs, SNSs, or HNP expression plasmids. At 24 h posttransfection, cells were left untreated (A and C) or treated with IFN-α (1000 units/ml) (B and D) for 30 min. After fixation, subcellular localization of the indicated viral proteins and STAT2 (A and B) or STAT1 (C and D) was visualized by IFA and confocal microscopy. Nuclei were stained with Hoechst.