Figure 7.

HRTV infection interferes with type I and III IFN-triggered nuclear translocation of both STAT2 and STAT1. HEK293 cells infected with HRTV (MOI = 1) or mock-infected were left untreated (A) or treated with IFN-α (1000 units/ml) (B) or IFN-λ (100 ng/ml) (C) for 30 min at 24 hpi. After fixation, IFA was performed to visualize the subcellular localization of HNSs and STAT2 or STAT1 under a confocal microscope. Nuclei were stained with Hoechst. D and E, cells mock-infected or infected with HRTV (HNSs-positive) from the experiments of B and C were scored for STAT2 (D) or STAT1 (E) nuclear accumulation, respectively. For each group, ∼100 cells were counted. Percentages of the cells with noticeable STAT2 or STAT1 nuclear accumulation were calculated, respectively. Data are presented as mean ± S.D. (error bars) (n = 3). Also see Fig. S1.