Table 1.
Expression plasmids used in the DRaCALA assay
| Plasmid | Protein | Functiona | Domain |
|---|---|---|---|
| pGP2594 | KtrA | Peripheral membrane component K+ transporter | RCK_C |
| pGP2906 | KhtT | K+/H+ antiporter | RCK_C |
| pGP2907 | KtrC | Peripheral membrane component K+ transporter | RCK_C |
| pGP2908 | YrvC | Unknown | RCK_C |
| pGP2908 | YjbQ/CpaA | Unknown | RCK_C |
| pGP2913 | KimA | K+ transporter | b |
| pGP2922 | AcuB | Unknown | CBS |
| pGP2923 | CcpN | Transcriptional repressor | CBS |
| pGP2924 | OpuAA | Glycine betaine ABC transporter (ATP-binding protein) | CBS |
| pGP2927 | MgtE | Primary Mg2+ transporter | CBS |
| pGP2928 | YhdT | Unknown | CBS |
| pGP2929 | YkuL/DarB | Unknown | CBS |
| pGP2930 | YhcV | Unknown, forespore-specific sporulation protein | CBS |
| pGP2931 | YhdP | Potential Mg2+ efflux pump | CBS |
| pGP2932 | YrkA | Unknown | CBS |
| pGP2933 | YtoI | Unknown, similar to transcriptional regulator (GntR family) | CBS |
| pGP2934 | YlbB | Unknown, putative oxidoreductase | CBS |
| pGP2935 | YqhB | General stress protein | CBS |
| pGP2936 | YugS | Unknown | CBS |
| pGP2937 | GuaB | Biosynthesis of GMP | CBS |
| pGP2938 | OpuBA | Choline ABC transporter (ATP-binding protein) | CBS |
| pGP2939 | OpuCA | Glycine betaine/carnitine/choline ABC transporter (ABC-binding protein) | CBS |
a Protein functions have been retrieved from the SubtiWiki database (29).
b For KimA, no conserved c-di-AMP–binding domain has been identified so far.