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. 2019 Apr 29;294(24):9416–9429. doi: 10.1074/jbc.RA119.007485

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© 2019 Pandey et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 5. — C5a/C5a^pep-induced ERK1/2 phosphorylation is sensitive to PTX treatment. A, HEK-293 cells stably expressing C5aR1 were incubated with 100 ng/ml PTX for 12–16 h followed by serum starvation and ligand stimulation. Subsequently, ERK1/2 phosphorylation was measured by Western blotting. B, densitometry-based quantification of data presented in A from three independent experiments normalized with respect to maximal response (treated as 100%) and analyzed using one-way ANOVA. ***, p < 0.001. C, effect of PTX treatment on C5a^pep-induced ERK1/2 phosphorylation measured as in A above. D, densitometry-based quantification of data presented in C, normalized, and analyzed as in B.