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. 2019 Apr 29;294(24):9416–9429. doi: 10.1074/jbc.RA119.007485

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© 2019 Pandey et al.

Published under exclusive license by The American Society for Biochemistry and Molecular Biology, Inc.

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Figure 7. — C5a^pep induces differential recruitment of βarr1 and 2 at C5aV₂R. A, HEK-293 cells expressing FLAG-C5a-V2R and βarr2 were stimulated with saturating concentrations of different ligands followed by cross-linking using DSP. Subsequently, FLAG-C5a-V2R was immunoprecipitated using anti-FLAG antibody agarose, and co-elution of βarr2 was visualized using Western blotting. B, densitometry-based quantification of data presented in A (average±S.E.; n = 2) normalized with respect to signal for C5a-βarr2 30 min condition (treated as 100%) and analyzed using two-way ANOVA. **, p < 0.01; *, p < 0.05. C, interaction of C5aR1 or C5a-V2₂R with βarr1 measured essentially in similar fashion as described in A above. D, densitometry-based quantification of data presented in C (averages ± S.E.; n = 3) normalized with respect to signal for C5a-C5aR1 condition (treated as 100%) and analyzed using two-way ANOVA. **, p < 0.01.