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. 2019 May 6;294(24):9642–9654. doi: 10.1074/jbc.RA119.008419

Figure 3.

Figure 3.

The siRNA-mediated down-regulation of KDM5A inhibits 3T3-L1 preadipocyte differentiation. 3T3-L1 preadipocyte was transfected with control siRNA (siNC) or KDM5A siRNAs (siKDM5A-1 and siKDM5A-2) and induced to differentiation. A, on day 6 after adipogenic induction, cells were stained with Oil Red O, and representative images are shown. Scale bar, 100 μm. B, on Day 4 after adipogenic induction, the mRNA level of the indicated genes was determined by RT-qPCR. Data were normalized to the mock group. C, on day 4 after adipogenic induction, the indicated proteins were detected by Western blotting. Hsp90 is the loading control. D, at the indicated time point, the mRNA level of Fabp4 was determined by RT-qPCR. Data were normalized to data of day 0. All values are represented as means with error bars representing S.D. *, p < 0.05; ***, p < 0.001. Mock, cells were not infected with viruses or transfected with siRNAs. For statistical analysis, two-way analysis of variance and Bonferroni's post hoc tests were performed in B (compared with siNC) and D (compared with siNC).