Figure S6.

Worms that Are Homozygous for the hrde-1 Mutant Allele pig4 Are Defective in RNAi Inheritance, Related to STAR Methods

Worms heterozygote to the indicated genotype and expressing a germline GFP reporter were exposed to bacteria expressing anti-GFP dsRNA (P0 generation). We used heterozygotes so that the worms could initiate an RNAi response (rde-4 mutants do not initiate RNAi responses). GFP silencing levels were tested in the P0 heterozygotes and their homozygote mutated F2 progeny. These experiments were conducted in three independent replicates (n = > 30).

(A) Relative GFP (GFP/empty vector) fluorescence in F2 homozygotes. Each dot represents one worm. P values were determined by one-way ANOVA and Tukey’s post hoc correction for multiple comparison. ^∗∗∗∗- p < 10−4; n.s.- p > 0.05. Data shown are means ± SD.

(B) Relative GFP fluorescence levels (GFP/empty vector) between worm populations exposed to GFP RNAi at the P0 generation were averaged across trials.