Table 4. Strains and plasmids.
| Yeast strains | Source |
|---|---|
| BY4741 yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0) | Invitrogen |
| BY4742 yeast (MATα his3Δ1 leu2Δ0 lys2Δ0 ura3Δ0) | Invitrogen |
| Apq12Δ; Y01433 (MATa; ura3Δ0; leu2Δ0; his3Δ1; met15Δ0; YIL040w::kanMX4) | This study |
| W303 Apq12Δ apq12::hphNTI leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 | This study |
| Nup116-GFPboundary MKY227 (W303, ADE2+) | Mattheyses et al., 2010 |
| Nup116-GFPboundary BY4741 | This study |
| Apq12-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Apq12-GFP::His3M × 6) | ThermoFisher (Huh et al., 2003) |
| Nup2-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Nup2-GFP::His3M × 6) | ThermoFisher |
| Nup49-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Nup49-GFP::His3M × 6) | ThermoFisher |
| Nup100-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Nup100-GFP::His3M × 6) | ThermoFisher |
| Nup133-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Nup133-GFP::His3M × 6) | ThermoFisher |
| Heh2-GFP yeast (MATa his3 leu2 met15 ura3Δ0 Heh2-GFP::His3M × 6) | ThermoFisher |
| Srm1-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Srm1-GFP::His3M × 6) | ThermoFisher |
| Kap95-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Kap95-GFP::His3M × 6) | ThermoFisher |
| Crm1-GFP yeast (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Crm1-GFP::His3M × 6) | ThermoFisher |
| Msn2-GFP yeast (MATa his3 leu2 met15 ura3Δ0 Msn2-GFP::His3M × 6) | ThermoFisher |
| JTY7; Nup49-mCh (MATα Nup49-mCh::CaURA3 can1Δ::STE2pr-LEU2 ura3Δ0 lyp1Δ leu2Δ0 his3Δ1 met15Δ0) | Tkach et al., 2012 |
| yIS010; Nup2-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Nup2-GFP::His3M × 6 Nup49-mCh::URA) | This study |
| yIS011; Nup100-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0
met15Δ0 ura3Δ0 Nup100-GFP::Hi3M × 6 s Nup49-mCh::URA) |
This study |
| yIS012; Nup116-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Nup116-GFPboundary Nup49-mCh::URA) | This study |
| yIS013; Nup133-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Nup133-GFP::His Nup49-mCh::URA) | This study |
| yIS014; Nup49-GFP Nup133-mCh (MATa his3Δ1 leu2Δ0
met15Δ0 ura3Δ0 Nup49-GFP::His3M × 6 Nup133-mCh::URA) |
This study |
| yIS018; Apq12-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Apq12-GFP::His3M × 6 Nup49-mCh::URA) | This study |
| yIS021; Srm1-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Srm1-GFP::His3M × 6 Nup49-mCh::URA) | This study |
| yIS022; Kap95-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Kap95-GFP::His3M × 6 Nup49-mCh::URA) | This study |
| yIS023; Crm1-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Crm1-GFP::His3M × 6 Nup49-mCh::URA) | This study |
| yIS027; Apq12Δ Nup49-mCh GFP-NLS (MATa; ura3Δ0; leu2Δ0; his3Δ1; met15Δ0; YIL040wΔ::kanMX4; GFP-tcNLS(pGal1)::His Nup49-mCH::URA) | This study |
| yIS028; Apq12Δ Nup49-mCh GFP-NES (MATa; ura3Δ0; leu2Δ0; his3Δ1; met15Δ0; YIL040wΔ::kanMX4; GFP-NES(pGal1)::His Nup49-mCH::URA) | This study |
| yIS032; Chm7-yeGFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Chm7-yeGFP::His Nup49-mCh::URA) | This study |
| yIS035; Heh2-GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 Heh2-GFP::His3M × 6 Nup49-mCh::URA) | This study |
| yPP008; GFP-tcNLS Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 GFP-tcNLS(pGal1)::His Nup49-mCh::URA) | This study |
| yPP009; GFP Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 GFP(pGal1)::His Nup49-mCh::URA) | This study |
| yPP011; GFP-NES Nup49-mCh (MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 GFP-NES(pGal1)::His Nup49-mCh::URA) | This study |
| yAA001; Nab2NLS-GFP Nup49-mCh (MATα Nup49-mCh::CaURA3 can1Δ::STE2pr-LEU2 ura3Δ0 lyp1Δ leu2Δ0 his3Δ1:: Nab2NLS(pTpi1)::His met15Δ0) | This study |
| yAA002; Pho4NLS-GFP Nup49-mCh (MATα Nup49-mCh::CaURA3 can1Δ::STE2pr-LEU2 ura3Δ0 lyp1Δ leu2Δ0 his3Δ1::
Pho4NLS(pTpi1)::His met15Δ0) |
This study |
| Oligonucleotides | |
| Chm7-GFP-S3 fw (GAAAACCACGATAATGAGATAAGAAAAATCATGATGGAAGAACAACCACGTCGTACGCTGCAGGTCGAC) | This study |
| Chm7-GFP-S2 rv (CATATTTATTTTTTATTTATACATATATATTTATTTATTAGTCACTCAGTTCGATCGATGAATTCGAGCTCG) | This study |
| Plasmids | |
| Plasmid: pYM44 yeGFP-tag | Janke et al., 2004 |
| Plasmid: pYM28 EGFP-tag | Janke et al., 2004 |
| Plasmid: pNZ-h2NLS-L-GFP (ID-GFP) | Meinema et al., 2011 |
| Plasmid: pBT016 pYX242-NAB2NLS-GFP-PRA | Timney et al., 2006 |
| Plasmid: pBT018 pYX242-PHO4NLS-GFP-PRA | Timney et al., 2006 |
| Plasmid: pPP014 mCh-Ura-Cassette | This study |
| Plasmid: pPP042 pRS303-GFP-tcNLS | This study |
| Plasmid: pPP043 pRS303-GFP | This study |
| Plasmid: pPP046 pRS303-GFP-NES | This study |
| Plasmid: pAA8 pRS303-Nab2NLS-GFP | This study |
| Plasmid: pAA9 pRS303-Pho4NLS-GFP | This study |