Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (HEK293T) | HEK293T | ATCC | Cat:CRL-11268 | |
| Transfected construct (Human) | pBIND-RORγHinge-LBD | Kumar et al., 2010 DOI:10.1124/mol.109.060905 | Addgene_128091 | |
| Transfected construct (Human) |
pGL4.35[luc2P/9XGAL4 UAS/Hygro] Vector | Promega | Cat:E1370 | |
| Transfected construct (Human) | pSPORTV6-hRORγ | Kumar et al., 2012 DOI:10.1021/cb200496y | Addgene_128093 | |
| Transfected construct (Human) | pGLA4.1-5xRORE- Luciferase |
Kumar et al., 2012 DOI:10.1021/cb200496y | Addgene_128094 | |
| Commercial assay or kit | Britelite Luciferase assay reagent |
Perkin Elmer | Cat:6066766 | |
| Commercial assay or kit | Dual-Glo Luciferase assay reagents |
Promega | Cat:E2920 | |
| Recombinant DNA reagent | pESUMO-RORγLBD | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | Addgene_128090 | |
| Recombinant DNA reagent | pESUMO-RORγLBD-SRC2 | This Paper | Addgene_128089 | |
| Commercial assay or kit | Q5 mutagenesis kit |
New England Biolabs | Cat:E0554S | |
| Sequence-based reagent | Q5_hRORC_F378A_Sense | Sigma | N/A | CACGGTCTTTgcgGAAGGCAAATAC |
| Sequence-based reagent | Q5_hRORC_F378A_Antisense | Sigma | N/A | CGGTTGTCAGCATTGTAG |
| Sequence-based reagent | Q5_hRORC_F388A_Sense | Sigma | N/A | CATGGAGCTGgcgCGAGCCTTGG |
| Sequence-based reagent | Q5_hRORC_F388A_Antisense | Sigma | N/A | CCACCGTATTTGCCTTCA |
| Sequence-based reagent | Q5_hRORC_F486A_Sense | Sigma | N/A | GCTGCAGATCgcgCAGCACCTCC |
| Sequence-based reagent | Q5_hRORC_F486A_Antisense | Sigma | N/A | CTTTCCACATGCTGGCTAC |
| Sequence-based reagent | Q5_hRORC_K503A_Sense | Sigma | N/A | TCCACTCTACgcgGAGCTCTTCAGCACTG |
| Sequence-based reagent | Q5_hRORC_K503A_Antisense | Sigma | N/A | GGGAAAGCGGCTTGGACC |
| Sequence-based reagent | Q5_hRORC_K503R_Sense | Sigma | N/A | TCCACTCTACcgtGAGCTCTTCAGCACTGAAAC |
| Sequence-based reagent | Q5_hRORC_K503R_Antisense | Sigma | N/A | GGGAAAGCGGCTTGGACC |
| Sequence-based reagent | Q5_hRORC_F506A_Sense | Sigma | N/A | CAAGGAGCTCgcgAGCACTGAAACC |
| Sequence-based reagent | Q5_hRORC_F506A_Antisense | Sigma | N/A | TAGAGTGGAGGGAAAGCG |
| Sequence-based reagent | Q5_hRORC_Y502F_Sense | Sigma | N/A | caagGAGCTCTTCAGCACTGAAACC |
| Sequence-based reagent | Q5_hRORC_Y502F_Antisense | Sigma | N/A | aagagTGGAGGGAAAGCGGCTTG |
| Sequence-based reagent | Q5_hRORC_A368V_Sense | Sigma | N/A | GATGTGCCGGgtgTACAATGCTGAC |
| Sequence-based reagent | Q5_hRORC_A368V_Antisense | Sigma | N/A | CTAACCAGCACCACTTCC |
| Sequence-based reagent | Q5_hRORC_K354A_Sense | Sigma | N/A | TGTGCTTCTCgccGCAGGAGCAATG |
| Sequence-based reagent | Q5_hRORC_K354A_Antisense | Sigma | N/A | ATCTGGTCATTCTGGCAG |
| Sequence-based reagent | Q5_hRORC_K354R_Sense | Sigma | N/A | TGTGCTTCTCcggGCAGGAGCAATG |
| Sequence-based reagent | Q5_hRORC_K354R_Antisense | Sigma | N/A | ATCTGGTCATTCTGGCAG |
| Peptide, recombinant protein | Biotin-Linker-SRC1-2 | Lifetein | N/A | Biotin-Ahx-SPSSHSSLTERHKILHRLLQEGSP |
| Peptide, recombinant protein | HisSUMO-hRORgLBD(265-518) | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | N/A | |
| Peptide, recombinant protein | RORgLBD(265-507) | This Paper | N/A | For co-crystallography with SR2211 |
| Peptide, recombinant protein | RORgLBD(265-507)-linker-SRC2-2 | This Paper | N/A | For co-crystallography with SR19265 and SR19355 |
| Commercial assay or kit |
AlphaLISA Anti-HIS-Acceptor beads | Perkin Elmer | Cat:AL128C | |
| Commercial assay or kit | AlphaScreen Streptavidin-Donor beads | Perkin Elmer | Cat:6760002S | |
| Chemical compound, drug | Fc1cccc(Cl)c1OCc2ccc3ccn(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR18862 | |
| Chemical compound, drug | Fc1cccc(Cl)c1OCc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR18957 | |
| Chemical compound, drug | Fc1cccc(Cl)c1CCc2 ccc3ccn(c3c2)S(=O)(=O) c4cccc(c4)C(F)(F)F |
Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19081 | |
| Chemical compound, drug | Fc1cccc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19170 | |
| Chemical compound, drug | FC(F)(F)c1cccc(c1)S(=O)(=O)N2CCc3ccc(OCc4c(Cl)cccc4Cl)cc23 | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19171 | |
| Chemical compound, drug | Cc1cccc(Br)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19172 | |
| Chemical compound, drug | Cc1cccc(C)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19173 | |
| Chemical compound, drug | COc1ccccc1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19174 | |
| Chemical compound, drug | Fc1cccc(Cl)c1COc2ccc3ccn(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19257 | |
| Chemical compound, drug | Cc1cccc(c1)S(=O) (=O)N2CCc3ccc(OCc4c(F)cccc4Cl)cc23 |
Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19258 | |
| Chemical compound, drug | COc1cccc(c1)S(=O)(=O)N2CCc3ccc(OCc4c(F)cccc4Cl)cc23 | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19263 | |
| Chemical compound, drug |
Fc1cccc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cc(Cl)cc(Cl)c4 | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19264 | |
| Chemical compound, drug | FC(F)(F)c1cccc(c1)S(=O)(=O)N2CCc3ccc(OCc4c(Cl)cncc4Cl)cc23 | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19265 | |
| Chemical compound, drug | COc1cccnc1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19266 | |
| Chemical compound, drug | Fc1cccc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cncc(c4)C(F)(F)F |
Doebelin et al., 2016
DOI:10.1002/cmdc.201600491 |
ID:SR19267 | |
| Chemical compound, drug | FC(F)(F)Oc1ccccc1COc2ccc3CCN(c3c2) S(=O)(=O)c4cccc(c4)C(F)(F)F |
Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19268 | |
| Chemical compound, drug | CC(Oc1ccc2CCN(c2c1)S(=O)(=O)c3cccc(c3)C(F)(F)F)c4c(F)cccc4Cl | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19269 | |
| Chemical compound, drug | Fc1cccc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(n4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19344 | |
| Chemical compound, drug | Fc1cccc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(Cl)c4 | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19346 | |
| Chemical compound, drug | Fc1cccc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cc(ccn4)C(F)(F)F |
Doebelin et al., 2016
DOI:10.1002/cmdc.201600491 |
ID:SR19348 | |
| Chemical compound, drug |
Fc1cccc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4ccnc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19349 | |
| Chemical compound, drug | COc1cc(Cl)ccc1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19350 | |
| Chemical compound, drug | COc1c(Cl)cccc1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19351 | |
| Chemical compound, drug | FC(F)(F)c1cccc(c1)S(=O)(=O)N2CCc3ccc(OCc4c(Cl) ccnc4Cl)cc23 |
Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19352 | |
| Chemical compound, drug | COc1ccc(Cl)cc1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19353 | |
| Chemical compound, drug | Fc1cccc(Cl)c1C(=O)Nc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19355 | |
| Chemical compound, drug | CN(C(=O)c1c(F)cccc1Cl)c2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19379 | |
| Chemical compound, drug | Fc1cccc(Cl)c1CNc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19381 | |
| Chemical compound, drug | CN(Cc1c(F)cccc1Cl)c2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19382 | |
| Chemical compound, drug | CC1Cc2ccc(OCc3c(F)cccc3Cl)cc2N1S(=O)(=O)c4cccc(c4)C(F)(F)F |
Doebelin et al., 2016
DOI:10.1002/cmdc.201600491 |
ID:SR19425 | |
| Chemical compound, drug | Fc1cncc(Cl)c1COc2ccc3CCN(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19497 | |
| Chemical compound, drug | FC(F)(F)c1cccc(c1)S(=O)(=O)N2CCc3ccc(OCc4c(Cl)cncc4C(F)(F)F)cc23 | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19498 | |
| Chemical compound, drug | FC(F)(F)C1Cc2ccc(OCc3c(Cl)cncc3Cl)cc2N1S(=O)(=O)c4cccc(c4)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19546 | |
| Chemical compound, drug | Fc1cccc(Cl)c1COc2ccc3CC(N(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F)C(F)(F)F | Doebelin et al., 2016 DOI:10.1002/cmdc.201600491 | ID:SR19547 | |
| Chemical compound, drug |
Fc1cncc(Cl)c1COc2ccc3CC(N(c3c2)S(=O)(=O)c4cccc(c4)C(F)(F)F)C(F)(F)F |
Doebelin et al., 2016
DOI:10.1002/cmdc.201600491 |
ID:SR19548 | |
| Software, algorithm | HDX-Workbench | Pascal et al., 2012 DOI:10.1007/s13361-012-0419-6 | N/A | |
| Software, algorithm | Prism 5 | GraphPad | N/A | |
| Software, algorithm | R Studio | R studio Team (2015) | N/A | |
| Software, algorithm | PHENIX | Adams et al., 2010 DOI:10.1107/S0907444909052925 | N/A | |
| Software, algorithm | iMosflm | Battye et al., 2011DOI:10.1107/s0907444910048675 | N/A |