Table 1. Cryo-EM data collection, refinement and validation statistics.
| Structure of the 5’ ETS RNP PDB 6ND4 EMD-0441 |
|
|---|---|
| Data collection and processing | |
| Voltage (kV) | 300 |
| Pixel size (Å) | 1.6 |
| Electron exposure (e- / Å2) | 31.25 |
| Defocus range (um) | 1–3.5 |
| Symmetry imposed | C1 |
| Initial particle images | 275’080 |
| Final particle images | 52’629 |
| Resolution (Å) | 4.3 |
| FSC threshold | 0.143 |
| Map sharpening B-Factor (Å2 ) | −64.77 |
| Refinement | |
| Initial model used | 5WLC |
| Model composition | |
| Non hydrogen atoms | 64,019 |
| Protein residues | 10309 |
| RNA bases | 569 |
| Ligands | 0 |
| R.m.s. deviations | |
| Bond length (Å) | 0.01 |
| Angles (°) | 1.25 |
| Validation | |
| MolProbity score | 1.71 |
| Clashscore | 5.26 |
| Poor rotamers (%) | 0.0 |
| Good sugar puckers (%) | 98.7 |
| Ramachandran | |
| Favored (%) | 93.39 |
| Allowed (%) | 6.56 |
| Outliers (%) | 0.05 |