Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Homo sapiens) | Human SETD8 catalytic domain | Blum et al., 2014 | Uniprot: Q9NQR1-1 (positions 232–393) | with an N-terminal 6 × His tag in pHIS2, for in vitro assays and crystallography of APO. |
| Gene (Homo sapiens) | Human SETD8 catalytic domain | Ma et al., 2014 | Uniprot: Q9NQR1-1 (positions 232–393) | with an N-terminal 6 × His tag in pHIS2, for crystallography of BC-Inh2 and BC-SAM |
| Gene (Homo sapiens) | Human SETD8 catalytic domain | Addgene | Plasmid #51327 | with an N-terminal 6 × His tag in pHIS2, for crystallography of BC-Inh1 |
| Strain, strain background (E. coli) | Rosetta 2(DE3) | Novagen | #71400 | |
| Strain, strain background (E. coli) | BL21-CodonPlus(DE3)-RIL | Stratagene | #230245 | |
| Strain, strain background (E. coli) | BL21 (DE3) V2R-pRARE | SGC | ||
| Sequence-based reagent | Forward Primer for K382P | IDT | 5'-CTATGGGGACCGCAGCCCGGCTTCCATTGAAGCCC-3' | |
| Sequence-based reagent | Forward Primer for I293G | IDT | 5'-CGGGGACCTCATCGAGGGCACCGACGCCAAGAAAC-3' | |
| Sequence-based reagent | Forward Primer for E292G | IDT | 5'-CGGGGACCTCATCGGCATCACCGACGCCAAG-3' | |
| Peptide, recombinant protein | H4K20 peptide (10-30) | The Rockefeller University Proteomics Resource Center | NH2-LGKGGAKRHRKVLRDNIQGIT-COOH | |
| Chemical compound | SAM | Sigma Aldrich | #A2408 | |
| Chemical compound | [3H-Me]-SAM | PerkinElmer Life Sciences | #NET155001MC | |
| Commercial assay or kit | UltimaGold | PerkinElmer Life Sciences | #6013327 | |
| Software, algorithm | Anaconda Python | Oliphant, 2007; Millman and Aivazis, 2011 | ||
| Software, algorithm | ARP/wARP | Perrakis et al., 1997; Murshudov et al., 2011 | ||
| Software, algorithm | AUTOBUSTER | Emsley et al., 2010 | ||
| Software, algorithm | CCP4 suite | Collaborative Computational Project, Number 4, 1994 | ||
| Software, algorithm | COOT | Emsley and Cowtan, 2004 | ||
| Software, algorithm | Ensembler 1.0.5 | Parton et al., 2016 | ||
| Software, algorithm | Folding@home | Shirts and Pande, 2000 | ||
| Software, algorithm | GRADE | Bruno et al., 2004 | ||
| Software, algorithm | HKL2000 | PMID: 27799103 | ||
| Software, algorithm | IPython | Perez and Granger, 2007 | ||
| Software, algorithm | Jupyter Notebook | DOI: 10.3233/978-1-61499-649-1-87 | ||
| Software, algorithm | KinTek Explorer | Johnson et al., 2009 | ||
| Software, algorithm | matplotlib 2.2.2 | Hunter, 2007 | ||
| Software, algorithm | MDTraj | McGibbon et al., 2015a | ||
| Software, algorithm | MOGUL | Langer et al., 2008 | ||
| Software, algorithm | MolProbity | PMID: 20057044 | ||
| Software, algorithm | MOLREP | PMID: 20057045 | ||
| Software, algorithm | MSMBuilder | Harrigan et al., 2017 | ||
| Software, algorithm | MSMExplorer 1.1 | Harrigan et al., 2017 | ||
| Software, algorithm | NumPy | https://www.numpy.org | ||
| Software, algorithm | OpenMM 6.6.1 | Eastman et al., 2013 | ||
| Software, algorithm | Origin 7.0 | OriginLab | ||
| Software, algorithm | OriginPro 2018 | OriginLab | ||
| Software, algorithm | pandas | https://conference.scipy.org/proceedings/scipy2010/pdfs/mckinney.pdf | ||
| Software, algorithm | PDBFixer 1.3 | https://github.com/pandegroup/pdbfixer | ||
| Software, algorithm | PHASER | McCoy, 2007 | ||
| Software, algorithm | phenix.refine | Adams et al., 2010; Afonine et al., 2012 | ||
| Software, algorithm | POINTLESS/AIMLESS | Evans and Murshudov, 2013 | ||
| Software, algorithm | PRODRG | Schüttelkopf and van Aalten, 2004 | ||
| Software, algorithm | PyEMMA | Scherer et al., 2015 | ||
| Software, algorithm | PyMOL 1.8.4 | Schrödinger, LLC | ||
| Software, algorithm | REFMAC | Murshudov et al., 1997 | ||
| Software, algorithm | seaborn 0.8.1 | DOI: 10.5281/zenodo.883859 | ||
| Software, algorithm | MODELLER 9.16 | Sali and Blundell, 1993 | ||
| Software, algorithm | XDS | Kabsch, 2010 | ||
| Software, algorithm | XtalView | McRee, 1999 |