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. 2018 Oct 22;103(3):421–427. doi: 10.1136/bjophthalmol-2018-312630

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© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Neovascularisation and suspected lymphatics within failed corneal grafts. (A, B and D) Case ID #1: an 88 year-old male patient with failed graft. (C) Case ID #2: an 87-year-old female patient with failed graft. All nine cases of corneal graft failure were found to contain neovascularisation, which is demonstrated above on H&E (A and B). Blood vessels are outlined by a rectangular area shown at 20× magnification (A). Blood vessels are indicated by arrows at 40× magnification (B). Monocellular infiltrates were seen around the lumen of these vessels. All nine cases of corneal graft failure were found to contain luminal and elongated profiles via podoplanin antibody (D2-40) immunohistochemistry. Two immunoperoxidase images are provided above with podoplanin-antibody staining lymphatics denoted by arrows (C and D). Scale bars represent (A) 100 µm and (B–D) 50 µm.