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. 2018 Oct 22;103(3):421–427. doi: 10.1136/bjophthalmol-2018-312630

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© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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Fluorescence in situ hybridisation detection of lymphatic marker mRNAs. (A–C) Case ID #2: an 87-year-old female patient with a failed graft. (D–I) Case ID #5: a 30-year-old male patient with a failed graft. Probes recognising (A and D) podoplanin mRNA, (B, E, and G) LYVE-1 or (H) VEGFR-3 were used. (C, F and I) 4′,6-diamidino-2-phenylindole is also shown (blue). A merged image (C, F) illustrates podoplanin and LYVE-1 double positive regions (yellow). Another merged image (I) illustrates a LYVE-1 and VEGFR-3 double positive region (orange). Scale bars represent 30 µm.