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. 2019 Mar 28;78(6):826–836. doi: 10.1136/annrheumdis-2018-214786

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© Author(s) (or their employer(s)) 2019. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

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CircSERPINE2 alleviates OA in vivo. (A) Safranin-O/fast green staining of the cartilage in the indicated groups at 8 weeks after surgery. Scale bar=200 µm. ACLT-induced OA rabbits were injected with AAV negative control, WT AAV CircSERPINE2, or MUT AAV CircSERPINE2, and the degree of knee OA was evaluated by safranin-O/fast green staining (n=12). (B) OARSI scoring was performed according to staining results; *p<0.05. (C) Micro-CT images of OA rabbits. (D) FISH and IHC staining of ACLT-induced OA rabbits injected with AAV negative control, WT AAV CircSERPINE2 or MUT AAV CircSERPINE2. (E) WB for the expression of ERG, SOX9, catabolic enzymes (MMP-3 and MMP-13), and ECM composition (COL2A1) in rabbit cartilage tissues. the injection of WT AAV CircSERPINE2 alleviated the progression of OA and induced the expression of Col2a1 in the rabbit model of OA. Scale bar=100 µm. (F) Schematic of the working hypothesis. AAV, adeno-associated virus; ACLT, anterior cruciate ligament transection; ECM, extracellular matrix; ERG, E26 transformation-specific-related gene; FISH, fluorescence in situ hybridisation; IHC, immunohistochemistry; MUT mutant; OA, osteoarthritis; OARSI, Osteoarthritis Research Society International; TUNEL, terminal dexynucleotidyl transferase(TdT)-mediated dUTP nick end labeling; WB, western blotting; WT, wild type.