Key resources table.
| Reagent type | Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Chemical compound, drug | Cycloheximide | Sigma | C7698 | Materials and methods subsection: Yeast strains and growth |
| Chemical compound, drug | Rapamycin | Sigma | R8781 | Materials and methods subsection: Yeast strains and growth |
| Chemical compound, drug | Auxin | Sigma | 1288G | Materials and methods subsection: Yeast strains and growth |
| Chemical compound, drug | Diazaborine | Provided by H Bergler. Zisser et al., 2018 |
PMID: 29294095 | Materials and methods subsection: Yeast strains and growth |
| Commercial assay or kit | TruSeq ChIP Sample Preparation Kit | Illumina | IP-202-9001DOC | |
| Antibody, rabbit polyclonal | Anti-RNA polymerase II CTD repeat YSPTSPS (phospho S5) | Abcam | ab5131 | Rabbit polyclonal; (1 ug per ChIP (50 ml OD = O0.5) |
| Antibody, rabbit polyclonal | Anti-Rap1 | N/A Schawalder et al., 2004
(PMID:15616569) |
RRID: AB_2801428 | Rabbit polyclonal; (5 ul per ChIP (50 ml OD = O0.5) |
| Antibody | Anti-Rpl3 | Provided by Warner, 1977. | PMID: 9121443 | Results, Figure 5. Mouse monoclonal; 1:10 000 |
| Antibody | Anti-Rpl6 | Provided by O Gadal | Rabbit polyclonal; 1:10 000 | |
| Antibody | Anti-Rps8 | Provided by G Dieci | Rabbit polyclonal; 1:10 000 | |
| Antibody, rabbit polyclonal | antibody Ifh1 | N/A (Knight et al., 2014) PMID: 25085421 | AB_2801429 | Rabbit polyclonal (2 ul per ChIP (50 ml OD = O0.5) |
| Antibody, rabbit polyclonal | Antibody Fhl1 | N/A (Knight et al., 2014) PMID: 25085421 | AB_2801431 | Rabbit polyclonal (2 ul per ChIP (50 ml OD = O0.5) |
| Strain, strain background | Saccharomyces cerevisiae, W303 | W303: MATa/MATα leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15 | Thomas & Rothstein, 1989. PMID: 2645056. Experimental procedures, Strains. Table 1. | See Supplementary file 7 |
| Other | Primary sequence files | GEO | accession number GSE125226 | Materials and methods subsection: Plasmid construction |
| Recombinant DNA reagent | pFA6a-link-GFPEnvy-SpHis5 (plasmid) | Addgene | RRID: Addgene_60782 | Materials and methods subsection: Plasmid construction |
| Recombinant DNA reagent | RPL25-envyGFP-plasmid | This paper | Plasmid #1037 | Materials and methods subsection: Plasmid construction |
| Recombinant DNA reagent | pRS315-RPL25-eGFP | Milkereit et al. (2001) PMID: 11313466 | Materials and methods subsection: Plasmid constructions |