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. Author manuscript; available in PMC: 2019 Jun 17.
Published in final edited form as: Oncogene. 2018 Nov 19;38(12):2123–2134. doi: 10.1038/s41388-018-0565-9

Figure 3.

Figure 3.

EGFL6 induces EMT in breast cancer cells. a, Immunofluorescence staining of E-cadherin and vimentin in paired MDA-MB-231 and MDA-MB-231/shEGFL6 cells. Nuclei were visualized with DRAQ5 staining (blue). Representative images and quantitative results are shown. Scale bar, 20 μm. b, qRT-PCR analysis showed the gene expression of E-cadherin, vimentin, fibronectin, Twist and snai1 in T47D and T47D/shEGFL6 cells. c, qRT-PCR analysis showed the gene expression of E-cadherin, vimentin, N-cadherin and snai1 inMDA-MB-231 and MDA-MB-231/shEGFL6 cells. d, qRT-PCR analysis showed the gene expression of E-cadherin, vimentin, fibronectin, Twist and snai1 in MCF-7 and MCF-7/EGFL6 cells. e, Western blot detection of E-cadherin, Vimentin and snail in the paired T47D and T47D/shEGFL6, MDA-MB-231 and MDA-MB-231/shEGFL6, and MCF-7 and MCF-7/EGFL6 cells. *, p < 0.05. Error bar, standard deviation (SD).