EGFL6 mediates cancer stem cell population of breast cancer. a and b, T47D, T47D/shEGFL6 and MCF-7, MCF-7/EGFL6 cells were double stained with CD24 and CD44 antibody. Flow cytometry assay was used to analyze the population of CD24 negative and CD44 positive cells. Representative images and quantitative results were shown. c and d, qRT-PCR analysis indicated gene expression of cancer stem cell markers in T47D, T47D/shEGFL6 and MCF-7, MCF-7/EGFL6 cells. Quantitative results are shown. *, p < 0.05. Error bar, SD.