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Clustering and marker gene identification of day 4 EBs. (A) tSNE visualization of 8 distinct clusters in day 4 EBs. (B) Visualization of the expression of a marker genes for pluripotent cells (Zfp42), epiblast stem cells(Fgf5), neuroectoderm (Sox1), and primitive streak (T). (C) Heatmap of lineage marker gene expression for each of the eight day 4 clusters.
