Figure 3. Blocking C3b binding to gC2 and IgG Fc binding to gE2.
Sera were pooled from 10 mice per group, IgG was purified and 50μg/ml was tested for (a) blocking gC2 binding to C3b or (b) blocking gE2 binding to IgG Fc. Results shown are the mean and SEM of 4 determinations for (a) and 2–3 for (b). P values comparing LNP with CpG were performed using the two-tailed Mann-Whitney test, while P values comparing antigen alone with LNP or CpG were calculated using a two-tailed one-sample t-test adjusted for a hypothetical value of 1. *, p<0.05; ***, p<0.001; ns, not significant.