(a) Cumulative percent blocking of gD2 binding by each mouse serum to (a) five prototype MAbs that bind to neutralizing epitopes, (b) two prototype MAbs that bind to cell-to-cell spread epitopes, and (c) all seven prototype MAbs that bind to neutralizing or cell-to-cell spread epitopes. Error bars in (a–c) represent SEM. (d) Correlation of neutralizing antibody titers of mice immunized with gD2 LNP-2 (n=10) or gD2 CpG/alum (n=10) with the cumulative percent blocking of antibodies produced to the five neutralizing epitopes. (e) Correlation of plaque size of mice immunized with gD2 LNP-2 (n=10) or gD2 CpG/alum (n=10) with the cumulative percent blocking of antibodies produced to the two cell-to-cell spread epitopes. (f) The number of epitopes blocked by mice in the gD2 LNP-2 or gD2 CpG/alum group. **, p<0.01; P values for (a-c) were calculated by the two-tailed Mann-Whitney test, (d–e) were calculated using Spearman’s correlation, and (f) by the two-tailed Fisher’s exact test (p=0.008).