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. Author manuscript; available in PMC: 2020 Jun 10.
Published in final edited form as: J Control Release. 2019 Apr 9;303:1–11. doi: 10.1016/j.jconrel.2019.04.010

Figure 2. In vitro DNA-NP distribution and transgene expression in F98-based 3D spheroids.

Figure 2.

(A) Representative images showing F98 tumor spheroids (red; mKate) 5 hours after treatment with DNA-NP (cyan; Cy5). Scale bar = 250 μm. (B) Quantification of radial distribution of DNA-NP within the spheroids. The coordinates 0 and 1 indicate the spheroid edge and core, respectively. (C) Representative reporter transgene expression (green; ZsGreen) by DNA-NP carrying ZsGreenexpressing plasmids 48 hours after NP-treatment. (D) Quantification of ZsGreen transgene expression within the spheroids. Differences are statistically significant as indicated (*p < 0.05).