Table 1.

Physicochemical properties of DNA-NP.

	Hydrodynamic diameter ± SEM (nm)^a	PDI^a	ζ-Potential ± SEM (mV)^b	Hydrodynamic diameter ± SEM at 12 hours in aCSF (nm)^c	PDI at 12 hours in aCSF^c
CPN	50 ± 1	0.16	18.0 ± 0.2	212 ± 3	0.17
BPN^L	49 ± 1	0.19	7.0 ± 0.3	67 ± 6	0.16
BPN^H	48 ± 5	0.20	2.0 ± 0.1	56 ± 3	0.15

^a Hydrodynamic diameter and polydispersity index (PDI) were measured by dynamic light scattering (DLS) in 10 mM NaCl. Mean ± SEM (n ≥ 3).

^b ζ-potential was measured by laser Doppler anemometry in 10mM NaCl at pH 7.0. Mean ± SEM (n ≥ 3).

^c Hydrodynamic diameter and PDI were measured by DLS following a 12-hour incubation in aCSF at 37° C.