Fig. 7.

Reproducible Wnt reporter induction across multiple L-WRN CM batches.

(A) Graph of CellTiter-Glo data from HEK293 Wnt reporter cells cultured in HEK cell media diluted 1:1 with the indicated media, either HEK medium, primary culture medium (1 culture), or L-WRN CM. Data are presented as fold change (mean ± s.e.m.) relative to HEK medium; n = 3 independent experiments. Group comparisons were not significant by 1-way ANOVA and Tukey’s post test. (B) Graph of luciferase activity expressed as fold induction (mean ± s.e.m.) over HEK293 Wnt reporter cells treated with primary culture medium; n = 3 independent experiments. Cells were treated with the indicated final concentrations of a single batch of L-WRN CM or with 1 μg/mL or 0.1 μg/mL of recombinant Wnt3a as a positive control. (C) Graph of luciferase activity expressed as fold induction (mean ± s.e.m.) over HEK293 Wnt reporter cells treated with primary culture medium; n = 3 independent experiments. Cells were treated with a 5% final concentration of the L-WRN CM1–13 or TE batches with 1 μg/mL of recombinant Wnt3a. (B, C) *P < 0.05, ***P < 0.001, ****P < 0.0001 by 1-way ANOVA and Dunnett’s post test relative to 0.1 μg/mL rWnt3a group (B) or the average Stappenbeck CM value (which was 18, represented by dashed line) (C).