Figure 3.

The Open-State Structure Model Reconciles Several Experimental Observations for Orai Gating

(A) The change of solvent accessibility of key residues in the pore during gating.

(B) The E262 assists to maintain the rigidity of the selectivity filter by forming an atomic interaction network.

(C) The well-packed anti-parallel coiled-coil pair on neighboring TM4-ext in the closed state (left panel) moves away upon gating (right panel), whereas the coupling between TM3 and TM4 through the hydrophobic packing between L304 and two consecutive residues T246 and A247 remains intact.