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. 2019 Jun 17;10(7):481. doi: 10.1038/s41419-019-1719-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Liver histology (H&E) in WT and GsdmD^−/− (KO) liver at 6 h after hemorrhagic shock with resuscitation (HS/R) or acetaminophen overdose (APAP). Lower graphs show quantification of necrotic area. Each point represents mean values for an individual mouse across at least ten fields of view. Horizontal bar represents overall mean value. b Liver immunofluorescent TMR staining in WT and GsdmD^−/− liver at 6 h after HS/R or APAP. Red = TMR-positive cells; blue = nuclei; green = actin. c Lower graphs show quantification of TMR-positive nuclei as a percentage of total nuclei. Each point represents mean values for an individual mouse across at least ten fields of view. Horizontal bar represents overall mean value. Scale bar, 40 μm. 20× Magnification. Dotted lines in H&E images outline necrotic areas. All images are representative of each group. N = 4/group. *p < 0.05 between indicated groups; **p < 0.001 between the indicated groups