Fig. 4.

Western blots of full-length (pro-)caspase-8 (Pro-cas8) and cleaved caspase-8 (Cl-cas8) in whole-cell lysates from liver of WT and GsdmD^−/− (KO) mice a at 6 h after hemorrhagic shock with resuscitation (HS/R), or b at 6 h after acetaminophen overdose (APAP). Quantitation of band density was performed across at least three separate blots and mean ± SEM is shown in the graphs (see below blots). c 4-HNE (lipid peroxidation) staining (red) in the liver from WT and GsdmD^−/− mice at 6 h after HS/R or APAP (blue = nuclei, green = actin). d Western blots of full-length caspase-8 (Full-cas8) and cleaved caspase-8 (Cleaved-cas8) in whole-cell lysates from WT hepatocytes at 1 h after hydrogen peroxide (H₂O₂) treatment at concentrations of 0.2, 1 or 5 mM. e Western blots of full-length caspase-8 (Full-cas8) and cleaved caspase-8 (Cleaved-cas8) in whole-cell lysates from WT hepatocytes at 1 h after 5 mM H₂O₂ treatment and N-acetyl cysteine (NAC) antioxidant given at concentrations of 0–5 mM. GAPDH used as loading control. Western blot images representative of results from at least three repeats. Each lane on Westerns contains liver from one individual mouse. Immunofluorescent images representative of multiple fields across mice from the same experimental groups. N = 4–5/group. *p < 0.05 between the indicated groups