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. 2019 Jun 17;10(7):478. doi: 10.1038/s41419-019-1710-2

Fig. 4. Morphometric alterations caused by BMAA on striatal primary neurons and neural stem cells.

Fig. 4

The effects on BMAA treatment with 50 µM to 3 mM BMAA was investigated directly after 24 h exposure of 8 DIV primary neurons, while the exposed NSCs were allowed to differentiate for 7 days after the exposure. Representative images of cells immunostained with anti-β III-tubulin (green), anti-MAP2 (red), and DAPI (blue) (a, f). Morphometric analysis was conducted using an ImageXpress Micro XLS Widefield HCA System (Molecular Devices, Sunnyvale CA, USA), where images were automatically captured and analyzed with the SoftMax Pro Software. Neurite length (b, g), processes per cell (c, h), branches per cell (d, i), and cell body area (e, j), were determined. Values represent mean ± SD from three independent experiments, each with five to six replicates. Statistically significant differences from control are indicated as follow: ***p < 0.001; **p < 0.01 and *p < 0.05 (one-way ANOVA followed by Tukey–kramer test). Scale bar = 50 µm