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. 2019 Jun 11;7:135. doi: 10.3389/fbioe.2019.00135

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Copyright © 2019 Mobaraki, Abbasi, Omidian Vandchali, Ghaffari, Moztarzadeh and Mozafari.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The characteristics of ultra-thin amniotic membrane. (A) The thickness change of amniotic membrane after digestion with collagenase type IV for different time durations. (B) HandE and Masson trichrome staining of IAM, DAM, and UAM tissues (Bar: 100 μ m). (C) Macroscopic views of IAM, DAM, and UAM were evaluated by photography scanning in moist form and light microscope in freeze dry form. (D) Hoechst whole mount staining of IAM, DAM, and UAM (Bar: 100 μ m). Reprinted with permission from Zakaria et al. (2014).