Table 1.

Particle size and zeta potential of CLs and corresponding lipoplexes at various N/P ratios: DOTAP/DOPE (1:1), DOTAP/Cholesterol (1:1), DOTAP/DOPE/Cholesterol (1:1:2.1), 2 mol% of DSPE-PEG and 0.4 mol% of Chol-PEG. (A) Size and PDI, (B) Zeta potential

A) Size and PDI liposome composition	CLs Size (nm) PDI	Lipoplexes (NP3) Size (nm) PDI	Lipoplexes (NP5) Size (nm) PDI	Lipoplexes (NP7) Size (nm) PDI
DOTAP/DOPE	170±3.2 0.2±0.03	186±1.7 0.2±0.05	226±4.3 0.2±0.04	291±6.8 0.1±0.04
DOTAP/DOPE/DSPE-PEG	167±2.5 0.2±0.04	178±3.4 0.2±0.06	217±5.4 0.2±0.04	265±4.5 0.1±0.03
DOTAP/Cholesterol	145±2.6 0.1±0.03	176±2.5 0.2±0.06	238±3.5 0.1±0.05	281±5.6 0.2±0.03
DOTAP/Cholesterol/Chol-PEG	132±4.3 0.2±0.06	166±3.5 0.1±0.04	226±6.2 0.2±0.07	279±3.8 0.2±0.03
DOTAP/Cholesterol/DSPE-PEG	138±3.3 0.2±0.08	180±3.3 0.1±0.03	231±2.6 0.2±0.03	283±4.4 0.2±0.03
DOTAP/DOPE/Cholesterol	174±2.5 0.1±0.02	190±2.4 0.2±0.02	219±4.4 0.2±0.03	255±4.3 0.1±0.03
DOTAP/DOPE/Cholesterol/DSPE-PEG	171±3.4 0.2±0.06	186±2.6 0.1±0.07	218±3.5 0.1±0.02	253± 3.4 0.2±0.03
B) Zeta potential liposome composition	CLs Zeta (mV)	Lipoplexes (N/P 3) Zeta (mV)	Lipoplexes (N/P 5) Zeta (mV)	Lipoplexes (N/P 7) Zeta (mV)
DOTAP/DOPE	44±0.2	40±0.2	47±0.3	50±0.1
DOTAP/DOPE/DSPE-PEG	28±0.4	25±0.3	32±0.1	37±0.7
DOTAP/Cholesterol	40±0.3	37±0.6	42±0.1	50±0.3
DOTAP/Cholesterol/Chol-PEG	27±0.1	22±0.1	28±0.3	35±0.3
DOTAP/Cholesterol/DSPE-PEG	26±0.7	22±0.3	31±0.2	39±0.5
DOTAP/DOPE/Cholesterol	41±0.2	34±0.2	39±0.6	45±0.2
DOTAP/DOPE/Cholesterol/DSPE-PEG	35±0.4	28±0.3	34±0.1	39±0.6
DOTAP/DOPE/Cholesterol/Chol-PEG	38±0.3	30±0.2	37±0.4	43±0.3

Abbreviations: PDI, polydispersity index; DOTAP, (1,2-dioleoyl-3-trimethylammoniumpropane); DOPE, (dioleoylphosphatidylethanolamine); DSPE-PEG, (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy(polyethylene glycol)]).