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. 2019 Jun 11;10:1257. doi: 10.3389/fimmu.2019.01257

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Copyright © 2019 Meira, Santos, Santo, Vasconcelos, Orge, Nonaka, Barreto, Caria, Silva, Barbosa-Filho, Macambira, Moreira and Soares.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gene expression in the hearts of infected mice after BA5 or benznidazole treatment. Analysis of gene expression was performed by real-time qRT-PCR using cDNA samples prepared from mRNA extracted from hearts of naive and chronic Chagasic mice treated with vehicle (Saline), BA5 (10 mg/Kg) or Benznidazole (Bdz; 100 mg/Kg). (A) Tnfa, (B) Ifnɤ, (C) IL1b, (D) Tgfb, (E) Il10, (F) Lgals3, (G) FoxP3, (H) Tbet (I) Gata3 (J) Nos2, (K) Arg1 and (L) Chi3 gene expression. Values represent the means ± S.E.M. of 5 mice (naïve) or 10 mice (infected) per group. *P < 0.05 compared to vehicle-treated mice; ^#P < 0.05 compared to naive group. ^$P < 0.05 compared to benznidazole-treated group.