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. 2019 Jun 11;10:1291. doi: 10.3389/fimmu.2019.01291

Figure 1.

Figure 1

Flagellin similarly activates HIV-DC and HD-DC. 2 × 105 MDDC from healthy donors (HD-DC; n = 5) and HIV-infected patients (HIV-DC; n = 5) were stimulated with 5 μg/ml flagellin (FLG) for 24 hours. Viability, expression of characteristic DC surface markers (A) as well as TNF (B) and IL-12p70 (C) secretion were analyzed and compared between untreated (UN) and stimulated (FLG) conditions as well between HD-DC and HIV-DC groups 0.4 × 105 FLG-treated MDDC were cultured with 4 × 105 autologous lymphocytes (MDDC/lymphocytes ratio: 1/0) for 96 hours to detect IFN-γ production in CD4+ T lymphocytes (percentage of CD3+ CD4+ T IFN-γ+ cells) (D), or 120 hours to measure CD4+ T lymphocytes proliferation (percentage of CD3+ CD4+ T CSFElow cells) (E). Lymphocytes alone were used as a negative control (Neg). Data are reported as mean ± standard error. Multiple t-test (A) and Two-Way ANOVA test (B–E) were applied to compare conditions within a group (HIV-DC or HD-DC; *p < 0.05) and between groups (HIV-DC vs. HD-DC; §p < 0.05).