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. 2019 Jun 11;10:1291. doi: 10.3389/fimmu.2019.01291

Figure 5.

Figure 5

Flagellin significantly induces NLRC4 but not NLRP3 in HIV-DC. 2 × 105 MDDC from healthy donors (HD-DC; n = 5) (A) and HIV-infected patients (HIV-DC; n = 5) (B) were pretreated with 10 μM MCC-950 or 10 μM parthenolide (PTD), and then stimulated with 5 μg/ml FLG for 24 hours or 1 μg/mL LPS for 24 hours with or without 1 mM ATP for more 15 minutes. IL-1ß concentration was measured in culture supernatants. 2 × 105 MDDC from healthy donors (HD-DC; n = 3) (C) and HIV-infected patients (HIV-DC; n = 3) (D) were transduced with empty vector (scramble: Scr), shNLRC4 or shNLRP3, and then stimulated with 5 μg/ml FLG for 24 hours, or 1 μg/mL LPS for 24 hours with or without 1 mM ATP for more 15 minutes. IL-1ß concentration was measured in culture supernatants. Kruskal-Wallis test with multi comparison post-test was applied (treated vs. UN: *p < 0.05; LPS+ATP vs. LPS #p < 0.05; within each group: §p < 0.05; +MCC-950 vs. +PTD: $p < 0.05; shNLRC4 vs. shNLRP3: £p < 0.05; shNLRC4 vs. Scr: @p<0.05).