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. 2019 Jun 11;10:1325. doi: 10.3389/fimmu.2019.01325

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Copyright © 2019 Carenza, Calcaterra, Oriolo, Di Vito, Ubezio, Della Porta, Mavilio and Della Bella.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Surface expression of immune checkpoints on DC subsets upon exposure to pro-inflammatory and anti-inflammatory stimuli. The expression of PD-L1, ILT2, and TIM-3 was assessed on DCs after 5-h incubation of whole blood samples in the absence or presence of LPS, IMQ, and IL-10 either alone or in combination. The expression level of each inhibitory molecule was expressed as net MFI. Color legend for DC subsets as reported in legend to Figure 3. Data were obtained from 16 healthy donors. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001 vs. basal. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. cells treated with TLR-ligand only. Statistical significance calculated using the paired t-test.