Table 3.

Borrelia burgdorferi antigens detected in infected macaque serum samples using a combination of mass spectrometry and protein array.

High-potential biomarkers (3 or more identifications)
	Method	Sample	MS X-Correlation value or Array reactivity ranking (sample type)
DbpA (BB_A24)	Direct MS	Macaque KD91−1 and 2 week post infection	Range: 1.86–4.34
	Protein Array	Macaque KD91−6 week post infection	In top 25 IgG responses (macaque)
Fla (BB_0147)	IP/MS	InMad Immune sera KD91immunized (x3)—IP of in vivo lysates; KD89 T6 coupled-IP of in vivo lysates	Range: 1.83–5.90
	Direct MS	Macaque KD89−1week post-infection	2.79
	Protein Array	InMad immune sera−8 week post immunization	In top 25 IgM responses (mouse)
VIsE (BB_F0041)	IP/MS	Macaque KD89 antibodies coupled to beads—IP of macaque KD89 serum-2-week post-infection	2.36
	Direct MS	Macaque KG87−1 week post infection	2.62
	Protein Array	Macaque KD91−6 week post infection	In top 25 IgG responses (macaque) In top 35 IgM responses (macaque)
Potential Biomarkers (2 identifications)
p83/100 (BB_0744)	Direct MS	Macaque KD91−1 and 2 weeks post-infection (pooled)	2.45
	Protein Array	InMad immune sera−8 weeks post immunizations	In top 25 IgM responses (mouse)
BB_G31	IP/MS	InMad immune sera coupled to beads—IP of in vivo lysate	2.19
	Direct MS	Macaque KD91−1 and 2 week post-infection (pooled)	2.51
BB_J48	Direct MS	Macaque KD91−1 and 2 week post-infection (not pooled)	Range: 2.22–2.35

A range in X-correlation values reflects identification from multiple samples. X-correlation value is an indication of the alignment of the peptide detected with the predicted mass-to-charge ratio of the theoretical peptide as assessed by Sequest (cut-off for inclusion 1.8). The array reactivity ranking was a reflection of the florescent intensity on the array, sample type indicates if macaque sera or InMAD immune sera was used to probe the array.