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. 2019 Jun 17;7(12):e14146. doi: 10.14814/phy2.14146

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© 2019 The Authors. Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Infarct size in isolated perfused rat hearts with in situ remote ischemic preconditioning. Closed symbols: means ± SD. Circles represent female rat hearts, and triangles represent male rat hearts. Placebo+GI/R: placebo protocol in situ and 30‐min/120‐min global zero‐flow ischemia/reperfusion ex vivo; 1‐RIPC+GI/R: one hindlimb remote ischemic preconditioning in situ and 30‐min/120‐min global zero‐flow ischemia/reperfusion ex vivo; 2‐RIPC+GI/R: both hindlimbs ischemic preconditioning in situ and 30‐min/120‐min global zero‐flow ischemia/reperfusion ex vivo. *P < 0.001 versus placebo+GI/R, respectively; #P < 0.01 versus 1‐RIPC+GI/R, respectively; †P = 0.026 versus 1‐RIPC+GI/R_female; ‡P = 0.016 versus 1‐RIPC+GI/Rmale; (two‐way ANOVA and Fisher's least significant difference post hoc tests).