Table 1.
Examples of several signal peptides that perform well in gram-positive bacteria.
| Signal peptide | Recombinant protein | Host | Yield | Ranking | Reference | ||
|---|---|---|---|---|---|---|---|
| Signal sequence | Origin | Protein | Origin | ||||
| MKNMSCKLVVSVT LFFSFLTIGPLAHA |
B. subtilis, Epr | Cutinase | F. solani pisi | B. subtilis TEB1030 | 4.67 [U/mL] | 1/173 | Brockmeier et al., 2006 |
| MAKPLSKGGILVKKVLIAGA VGTAVLFGTLSSGIPGLPAADAQVAKA |
B. subtilis, YncM | Aminopeptidase | B. subtilis Zj016 | B. subtilis WB600 | 88.59 [U/mL] | 1/20 | Guan et al., 2016 |
| MKKFNFKTMLLLVLASCVFGVV VNVTTSLGPQTAITAQA |
L. plantarumWCFS1 | NucA (nuclease) | S. aureus | L. plantarum WCFS1 | 35.84 [U/mL] | 1/78 | Mathiesen et al., 2009 |
| MKEVRFWGLLLGL FVCLGAVIPLVSKA |
L. plantarumWCFS1 | AmyA (amylase) | L. amylovorus NRRL B-4549 | L. plantarum WCFS1 | 3.4 [102mU/mL] | 1/18 | Mathiesen et al., 2009 |
| MQINRRGFLKA TAGLATIGAASMFMPKANA |
C. glutamicum R | AmyE (α-amylase) | G. stearothermophilus | C. glutamicum | 288.3 [U/mL] | 1/31 | Watanabe et al., 2009 |
| MRSKKLWISLLF ALTLIFTMAFSNMSA |
B. licheniformis WX-02, AprE | Nattokinase | B. subtilis natto | B. licheniformis Δ0F-3 | 31.99 [FU/mL] | 1/81 | Cai et al., 2016 |
| MKNMSCKLVVSVTL FFSFLTIGPLAHA |
B. subtilis, Epr | Cutinase | F. solani pisi | C. glutamicum | 13.1 [U/mL] | 1/64 | Hemmerich et al., 2016 |
| MKKFPKKLLPIAVL SSIAFSSLASGSVPEASA |
B. subtilis, PhoB | XynBYG (alkaline active xylanase) | B. pumilus BYG | B. subtilis WB700 | 327.2 [U/mL] | 1/138 | Zhang et al., 2016 |
| MRSKKLWISLLFAL TLIFTMAFSNMSVQA |
B. subtilis168, AprE | Alkaline protease | B. alcalophilus TCCC11004 | B. subtilis WB600 | 7574.08 [U/mL] | 1/35 | Our lab |
| MRIFKKAVFVIMI SFLIATVNVNTAHA |
B. subtilis 168, DacB | Alkaline protease | B. alcalophilus TCCC11004 | B. amyloliquefaciens 111018 | 19835.7 [U/mL] | 1/86 | Our lab |