Figure 4.

Uptake of luminal latex beads by GP2⁺Tnfaip2⁺ cells in the trachea. (A) Latex beads (magenta) were dropped into the nasal cavity of C57BL/6N mice administered with GST or GST-RANKL as described in the method section. Immunofluorescence images of the trachea immunostained for Tnfaip2 (green). Bars: 100 μm. (B) Confocal images of the tracheal epithelium immunostained for Tnfaip2 (green) and GP2 (cyan). Orthogonal images from confocal images. (C) Quantification of the number of latex beads in the tracheal epithelia. Data expressed as mean ± standard deviation of the number of beads relative to the area of the epithelia (beads/μm²) were shown. Data were obtained from 4 mice in each group from 2 independent experiments. *P < 0.05 calculated with Student's t-test.