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. 2019 May 22;20(1):401–408. doi: 10.3892/mmr.2019.10267

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Copyright: © Nie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Effects of Cro on ROS and intracellular Ca²⁺ levels in Dex-treated MC3T3-E1 osteoblasts. Cells were pretreated with Cro (5, 25 and 100 µM) for 1 h and were then treated with 1 µM Dex for 24 h. (A) ROS levels, as determined by flow cytometry. (B) Quantitative analysis of ROS. (C) Intracellular Ca²⁺ levels, as determined by flow cytometry. (D) Quantitative analysis of intracellular Ca²⁺ levels. Data are presented as the means ± standard deviation of three independent experiments. ***P<0.001 vs. Ctrl; ^###P<0.001 vs. Dex. Cro, crocin; DCFH-DA, dichlorodihydrofluorescein diacetate; Dex, dexamethasone; ROS, reactive oxygen species.