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. 2019 May 22;20(1):655–663. doi: 10.3892/mmr.2019.10260

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Copyright: © Gao et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 6. — Effects of Zn on intracellular ROS in the HPMCs stimulated by HG. HPMCs were co-treated with HG, and TPEN or ZnSO4 for 48 h. (A) Intracellular ROS were measured by flow cytometry using an oxidation-sensitive fluorescent probe, dichlorodihydrofluorescein diacetate, which is oxidized to 2,7′-dichlorofluorescein in the presence of ROS. (B) Data are presented as the mean ± standard deviation. P-values were determined using ANOVA with Tukey's post hoc test. *P<0.05 vs. NC; #P<0.05 vs. HG. NC, negative control; HG, high glucose; TPEN, N,N,N',N'-tetrakis (2-pyridylmethyl) ethylenediamine; Zn, ZnSO4; HPMC, human peritoneal mesothelial cell; ROS, reactive oxygen species.