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. 2019 May 21;14:159–171. doi: 10.1016/j.omto.2019.05.003

Figure 1.

Figure 1

Phenotypic Differences among B5R Recombinant Viruses

(A) Schematic representation of the B5R recombinant VVs LC16mO (B5R+), LC16mO Δ-DsRed (B5R−), and LC16mO ΔSCR (SCR−). (B) RK13 cells were infected with the three B5R recombinant viruses, and viral plaques were photographed 48 h later. Top shows phase-contrast images, and bottom shows fluorescence micrographs of the same fields. Scale bar, 500 μm. (C) EEV and IMV were recovered from culture supernatants or lysates of A549 lung carcinoma cells infected with the three B5R recombinant viruses at an MOI of 0.1, and they were individually titrated in RK13 cells. Total PFU was calculated from plaque numbers and total volume of viral reagent. Data represent mean ± SD (n = 3). (D) Schematic representation of the VGF- and O1-deleted virus B5R LC16mO VGF−/O1− (B5R) and SCR-deleted virus LC16mO ΔSCR VGF−/O1− (ΔSCR).