Figure 5.

Neutralization Resistance of SCR-Deleted EEV or IMV against Anti-virus Antibody and Complement

(A) EEV in the culture supernatant of SKOV3 cells infected with B5R or ΔSCR was mixed with 0%, 0.2%, 0.5%, or 1% rabbit anti-VV-immunized serum (IgG fraction) and/or 0%, 1%, 3%, 10%, or 25% rabbit complement. The mixture was used to infect SKOV3 cells, which were photographed under a fluorescence microscope 96 h after infection. Scale bar, 1,500 μm. (B) Quantification of GFP fluorescence described in (A) as a function of serum (IgG) concentration. (C) IMV produced from lysates of SKOV3 cells infected with B5R or ΔSCR was mixed and used for SKOV3 cell infection as described (A). Images were acquired 96 h later. (D) Quantification of GFP fluorescence in (C). Data in (B) and (D) are presented as means ± SD (n = 3).