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. 2019 May 9;20(1):375–387. doi: 10.3892/mmr.2019.10223

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Copyright: © Shen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Effect of pressure on HepG2 and Huh-7 liver cancer cell migration and invasion in vitro. (A) HepG2 and (B) Huh-7 confluent cell monolayers were wounded and cultured under different levels of pressure (0, 5, 15, 30, 60 mmHg) for 24 h (magnification, ×100); (C) HepG2 and (D) Huh-7 percentage of migration was calculated. (E) Representative images of Transwell assay results showing cells stained with 0.1% crystal violet solution (magnification, ×100). Effect of pressure on HepG2 and Huh-7 liver cancer cell migration and invasion in vitro. The assay lasted 24 h, and after fixing and staining, the percentage of (F) HepG2 and (G) Huh-7 cells on the lower chamber was calculated; 10% acetic acid was used to elute the stained inserts to detect the percentage of invaded cells. mRNA expression levels of MMP1, MMP2, MMP7, MMP14 and TIMP1 in 3D-cultured (H) HepG2 and (I) Huh-7 cells exposed to different levels of pressure (0, 5, 15, 30, 60 mmHg) for 24 h. All data are expressed as the means ± standard deviation. The data were analyzed using ANOVA, followed by the least significant difference post hoc test. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 mmHg. ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. 15 mmHg. 2D, 2-dimensional; 3D, 3-dimensional; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinases; NS, not significant.

Figure 2. — Effect of pressure on HepG2 and Huh-7 liver cancer cell migration and invasion in vitro. (A) HepG2 and (B) Huh-7 confluent cell monolayers were wounded and cultured under different levels of pressure (0, 5, 15, 30, 60 mmHg) for 24 h (magnification, ×100); (C) HepG2 and (D) Huh-7 percentage of migration was calculated. (E) Representative images of Transwell assay results showing cells stained with 0.1% crystal violet solution (magnification, ×100). Effect of pressure on HepG2 and Huh-7 liver cancer cell migration and invasion in vitro. The assay lasted 24 h, and after fixing and staining, the percentage of (F) HepG2 and (G) Huh-7 cells on the lower chamber was calculated; 10% acetic acid was used to elute the stained inserts to detect the percentage of invaded cells. mRNA expression levels of MMP1, MMP2, MMP7, MMP14 and TIMP1 in 3D-cultured (H) HepG2 and (I) Huh-7 cells exposed to different levels of pressure (0, 5, 15, 30, 60 mmHg) for 24 h. All data are expressed as the means ± standard deviation. The data were analyzed using ANOVA, followed by the least significant difference post hoc test. *P<0.05, **P<0.01 and ***P<0.001 vs. 0 mmHg. ^#P<0.05, ^##P<0.01 and ^###P<0.001 vs. 15 mmHg. 2D, 2-dimensional; 3D, 3-dimensional; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinases; NS, not significant.