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. 2019 Jun 14;21(8):731–739. doi: 10.1016/j.neo.2019.05.003

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© 2019 The Authors

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

PMC Copyright notice

Effect of AZD2014 and rapamycin on EMT in solid tumors of Tsc2^+/− mice.

(A) IHC analysis. Adjacent kidney sections prepared from 16-month-old Tsc2^+/− mice treated with vehicle, AZD2014, or rapamycin were used to analyze EMT by IHC. Representative IHC-stained images were presented to show protein levels of vimentin, FSP1, and C-cadherin in solid tumors. Black lines are scale bars.

(B) Western blot analysis. Proteins were prepared from solid carcinomas dissected from Tsc2^+/− mice treated for 2 months from the age of 14 months with vehicle, AZD2014, or rapamycin. Beta-actin was used as a loading control. Representative Western blots were presented to show expression levels of vimentin and E-cadherin.