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MS-IHC.
Two adjacent kidney sections prepared from a Tsc2+/− mouse 14 months old were used for MS-IHC to stain vimentin. After stripping primary antibody against vimentin stained in the first round of IHC, no specific staining was observed in the second round of IHC with diluent instead of primary antibody against vimentin, but similar staining was observed in the third round of IHC with primary antibody against vimentin.
