Supplementary Figure 5.

Co-expression of epithelial and mesenchymal markers by MS-IHC in individual tumor cells of a renal lesion from a Tsc2^+/− mouse.

A kidney section prepared from a 16-month-old Tsc2^+/− mouse was used for MS-IHC. The same kidney section was subjected to three rounds of IHC to sequentially detect three indicated antigens. Representative images were presented to show examples of tumor cells co-expressing E-cadherin and vimentin/FSP1. Higher-power views of boxed areas are presented next to the corresponding lower-power images. Black lines are scale bars.