Supplementary Figure 7.

Effect of AZD2014 and rapamycin on mTORC1 and mTORC2 in the kidneys of Tsc2^+/− mice.

Western blot was used to examine mTORC1 and mTORC2 activity. Proteins were prepared from normal kidney tissues dissected from Tsc2^+/− mice treated for 2 months from the age of 14 months with vehicle, AZD2014, or rapamycin. Beta-actin was used as a loading control. Representative Western blots were presented to show phosphorylation of mTOR at S2448 and S2481, S6 at S235/236, Akt at T450 and S473, and PKCα at T638.