Skip to main content
. 2019 May 29;18(1):705–710. doi: 10.3892/etm.2019.7626

Figure 4.

Figure 4.

mRNA and protein expression of α-SMA and OPN via reverse transcription-quantitative polymerase chain reaction and western blot analysis. (A) In the normal co-culture groups, the mRNA expression of α-SMA was increased compared with the AR co-culture groups. (B) Furthermore, the mRNA expression of OPN in the AR co-culture groups was increased compared with the normal co-culture groups. (C) Western blot analysis indicated similar findings with regard to α-SMA and OPN protein expression in the normal +VSMCs and calcified +VSMCs co-culture groups. **P<0.05 and ***P<0.001 as indicated. OPN, osteopontin; α-SMA, α-smooth muscle actin. Normal + VSMCs, normal arterial decellularized scaffolds + VSMCs co-culture group; Calcified + VSMCs, calcified arterial decellularized scaffolds + VSMCs co-culture group.